Identification and characterization of strawberry FvGELP1 (Fragaria vesca GDSL esterase/lipase)

Abstract. Witasari LD, Huang FC, Schwab W. 2022. Identification and characterization of strawberry FvGELP1 (Fragaria vesca GDSL esterase/lipase).Biodiversitas 23: 907-915. Fruit softening is primarily the result hydrolytic enzymes activity. Esterases are reasonable candidates due to their putative role in cell wall components degradation. This study aimed identify characterize a new enzyme related fruit ripening. Esterase activity was detected native PAGE protein extract isolated from Fragaria ×ananassared fruit. Amino acid sequence analysis band revealed several esterases as possible candidates. esterase/lipase vesca(gene27964; FvGELP1) chosen for cloning purposes further analysis. Quantitative RT-PCR FvGELP1in plant tissues F. vescaindicated high expression levels fruit, particular early developmental stages. The gene FvGELP1(1,161 bp) amplified vescafruit cDNA expressed 43 kDa HisTag fusion Saccharomyces cerevisiae.FvGELP1 possesses four conserved residues Gly43-Asp44-Ser45-Asn46. contains Ser45, Asp364, His367 catalytic triad. assays resulted aromatic alcohol products by applying ?-naphthyl acetate (?NA), p-nitrophenyl (pNPA), phenyl acetate, benzyl substrates. It could be suspected that plays ripening might involved hemicellulose degradation, presumably deacetylation polysaccharide.